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果冻影院 Genomics is a collaborative research facility with expertise in state-of-the-art genomic technologies, project design and data analysis. Operational for over 20 years, this cost recovery, not-for-profit core facility uses the most up-to-date equipment to support your genomic research requirements. We are equipped automated systems for optimal high throughput and consistency and can accommodate both small and large scale projects.
Highly experienced application specialists offer full support at every stage from optimal experimental design to data analysis.听We support collaborations both nationally and internationally, including those in academia, clinical settings and industry.听
We form part of the Genetics and Genomic Medicine programme听at the 果冻影院 Great Ormond Street Institute of Child Health. 果冻影院 Genomics moved to dedicated laboratory space at the in 2019 and merged with 果冻影院 Pathogen Genomics Unit in January 2020 to create an expanded听facility, pooling both resources and expertise and now forms part of the听Genomics Science Technology platform at 果冻影院.
- About 果冻影院 Genomics
果冻影院 Genomics is housed at the听听with its own bespoke, dedicated laboratories.听Our facility听offers a number of dedicated genomic streams, including:
1. Nucleic acid extractions
2. Quality Control
3.听Genotyping microarrays
4. Methylation microarrays
5. Short/Long read sequencing
6. Pathogen sequencing
7. Single cell sequencing
8. Bioinformatics听
9.听Education听
- Technology at 果冻影院 Genomics
果冻影院 Genomics is a modern facility with state-of-the-art technologies. Our labs are equipped with a range of QC and microarray equipment, liquid handing automation and听sequencers. We have dedicated pre-PCR laboratories, dedicated genomic laboratory workspace and a genomic instrument laboratory.听Some technologies are accessible听for hire by 果冻影院 users following training. Below highlights some of our technologies at 果冻影院 Genomics.
A range of liquid handlers:
- Hamilton Nimbus Presto with Kingfisher technology (for high throughput extractions)
- Hamilton STAR and Hamilton STARlet
- Agilent Bravo liquid handler x2
- Opentrons OT-2听liquid handler
- Agilent Magnis (for low sample number, walk-away SureSelect library preparations)A range of sequencers including:
- MiniSeq, MiSeq x2, NextSeq 500, NextSeq 2000, MinION and GridIONA range of quality control equipment including:
- Qubit fluorometer (2.0 and 3.0), Agilent Bioanalyser 2100, Agilent Tapestation 4200 x2, Nanodrop and an Omega FLUOstar plate readerA range of equipment for high throughput microarray processing:
- TECAN听liquid handler, iScan with autoloader, Hybex and ovensA range of ancillary equipment including:
- Covaris, Vacuspeed, plate centrifuges, deep well centrifugeA range of equipment to support single cell technology
- Chromium single cell 10x, Octo dissociatorBioinformatics support
- Powerful clusters, IPA software听
Other genomic technologies at 果冻影院
Long Read Sequencing Facility
For access to PacBio and ONT platforms, please visit the Long Read Sequencing Facility at 果冻影院 Queen Square Institute of Neurology via this link /ion/research/research-departments/neuromuscular-diseases/long-read-sequencing-facility
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iSeq@果冻影院
The Illumina iSeq is a state-of-the-art, innovative and user-centric sequencing system offering accessible benchtop NGS sequencing that directly engages students and staff in the use of the technology. Experienced users can use the machine for preliminary or proof of concept experiments and students can gain hands-on experience in NGS sequencing coupled with high performance computing as part of their taught programmes of study, which is unparalled in a university. iSeq@果冻影院 is located within the Division of Biosciences. Please contact Prof Andrea Townsend-Nicholson for more information on accessing this instrumentation.
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- Our applications
Select one of the dedicated application streams below for more information
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- Education and training
Our expert application specialists are on hand to help design your projects, assist with grant applcations, walk you through genomic concepts, explain technologies and data formats.听果冻影院 Genomics also听offers routine training and access to听some of our equipment to allow you to run your own projects (access currently limited due to Covid-19 restrictions as of April 2022). Contact us for more information.
Equipment Open to all users Training required Charge for use/access Illumina NextSeq 2000 果冻影院 users听 Yes Yes Illumina NextSeq 500 果冻影院 users听 Yes Yes Illumina MiSeq 果冻影院 users听 Yes Yes Illumina MiniSeq 果冻影院 users听 Yes Yes Omega FLUOstar plate reader 果冻影院 users听 Yes Yes Agilent Tapestation 4200 果冻影院 users听 Yes Yes Agilent Bioanalyser 果冻影院 users听 Yes Yes Covaris E220 Focused-ultrasonicator 果冻影院 users听 Yes Yes QuantaBio qPCR machine 果冻影院 users听 Yes Yes ONT GridION 果冻影院 users听 Yes Yes 听
iSeq@果冻影院
The Illumina iSeq is a state-of-the-art, innovative and user-centric sequencing system offering accessible benchtop NGS sequencing that directly engages students and staff in the use of the technology. Experienced users can use the machine for preliminary or proof of concept experiments and students can gain hands-on experience in NGS sequencing coupled with high performance computing as part of their taught programmes of study, which is unparalled in a university. iSeq@果冻影院 is located within the Division of Biosciences. Please contact Prof Andrea Townsend-Nicholson for more information on accessing this instrumentation.
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Short course in Applied Genomics 2022
If you are interested in attending a short course in Applied Genomics, please get in contact with听Mark Kristiansen听to discuss. *Note that this course is extremely popular, and there may be a long waiting list.
Wednesday 12听October - Tuesday 18听October 2022听- FULL
This module aims to provide students with an up-to-date and broad knowledge of the principles of genomics, genomic technologies, methods of genomic analysis and the application of Genomics in medicine and life sciences.
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MSc Personalised Medicine and Novel Therapies
The Applied Genomics module is part of the Master's degree in Personalised Medicine and Novel Therapies听run from the 果冻影院 Great Ormond Street Institute of Child Health. This MSc programme provides an in-depth education in this cutting-edge and rapidly developing field. It is delivered by scientists and clinicians and includes a focussed set of core modules specialising in all aspects of personalised medicine, including genomics, bioinformatics, statistics and the regulatory aspects of novel therapies such as clinical trial design. Optional modules allow you to explore the applications and scope of personalised medicine. You are given opportunities to develop analytic skills during the programme before undertaking your independent research project. For more information, please click听here.
Click here for more information about the MSc Personalised Medicine and Novel Therapies programme at 果冻影院.
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MSc Cell and Gene Therapy
The Applied Genomics module is part of the Master's degree in Cell and Gene Therapy run from the 果冻影院 Great Ormond Street Institute of Child Health. This MSc programme provides an in-depth education in this cutting-edge and rapidly developing field. It is delivered by scientists and clinicians researching, developing and testing new treatments for genetically inherited and acquired diseases using gene delivery technology, stem cell manipulation and DNA repair techniques. For information, please click听here.
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Work experience
果冻影院 Genomics prides itself on providing in depth training in genomic technologies to scientists, researchers and clinicians as well as accommodating work experience students and hosting educational programmes as part of the听. Please听get in touch听for more information.
- FAQs
Here you will find answers to some of the most frequently asked questions
What can 果冻影院 Genomics do?
We offer a wide range of services including nucleic acid extractions, genotyping and methylation microarrays, DNA/RNA short and long read sequencing, pathogen sequencing and single cell sequencing.听For most听of our streams, we provide a complete service provision model from project design through to analysis (if required). We are happy to help with small projects and have the automation for larger high-throughput studies.听We can also provide training to 果冻影院 users to use some of our equipment.How much will it cost?
果冻影院 Genomics is a collaborative cost recovery core facility and we keep our costs to a minimum. We charge facility fees which cover the day-to-day costs fof operating the facility. These fees are based on the amount of time it will take us to complete your project. Our transparent quotes will clearly separate costs of the consumables and reagents and labware from the facility fee.听We are required to apply VAT (at 20%) on all projects from non-果冻影院 users of the facility. if you are an external collaborator to 果冻影院 Genomics and you have a collaboration with a 果冻影院 principal investigator that can be evidenced through a grant application, please do get in touch to discuss further details.How do I get a quote?
We will prepare a detailed quote for you based on your project needs. Please get in touch with the us to discuss your QC, extraction, microarray, RNA/DNA and single cell sequencing requirements. As we are a TRAC-listed facility, we will initially ask you to complete a Project Registration Form so that we can accurately quote for your project based on your funding source.How do I pay for the project?
You will first need to return a signed and dated quote.听For 果冻影院 users, we will arrange for the funds to be transferred from your 果冻影院 account through IDJ/IDT.听For external users, you will need to provide a purchase order (PO) with the quote and we will then invoice you for the work. Additional information may be required if you have not worked with 果冻影院 previously.What are the sample requirements for sequencing projects?
The sample requirements depend on the type of project. 听As a guide, our website has provided information about the听recommended input amounts for the kits we use most frequently.听The team will advise on the specific sample requirements when you are ready.How long will it take to process my samples?听
For sequencing projects, it is difficult to give accurate turnaround times for some听projects as this depends on the methodology used and how busy we are with other projects.听 Although听we aim to start processing your samples as soon as possible (and usually听within four听weeks of submission), sometimes there are external factors that are beyond our control (delivery delays, consumable shoratges). We will keep you informed if such a delay is foreseeable. In all cases, do听let us know if you have an urgent deadline for project completion. For samples submitted to the听methylation clinic, we aim to deliver data within four working days.
听What if I only want my samples QC鈥檇?
This option is available to 果冻影院 users only. 听Please get in touch with us and we will send you a request form.
As a general guide, we need 2碌L sample for both the Tapestation and Qubit.听
How will my data for sequencing projects be returned to me?
Internal 果冻影院 researchers will be provided with log-in details for a secure FTP site which allows them to access and download their data. Researcher external to 果冻影院 will receive access to a Globus Endpoint. This requires the install of a free plug-in which creates a secure connection to our servers. Data transfer can be optionally encrypted whilst transferring using OpenSSL AES256-SHA.For anonymised microarray data, 果冻影院 dropbox is the preferred choice of data transfer.
What sequencing data formats do you provide?
All data produced from your project belongs to you. For sequencing projects, we normally return demultiplexed FASTQ data. If you wish to perform your own demultiplexing, we can also provide Illumina BCL files or Nanopore FAST5. If we are also analysing data, we will supply you with QC reports, all aligned data and any final results, but this can vary depending on the analysis stream.
听What bioinformatic analysis can you provide for sequencing projects
We can provide analysis ranging from demutiplexing to custom analysis by our bioinformatician.听We also have standardised pipelines for alignment and differential gene expression analysis which we can run your data on and send you the results for interpretation.听For more in-depth and custom analysis, we will put you in touch with our bioinformatician to discuss your requirements. 听It鈥檚 best to do this before the project starts to make sure that the project design is suitable for the research question being asked.
听How long will you store data for?
We are unable to store data long term but will hold on to your data for three months after we have sent the raw or analysed data to you. After this time, this data will be deleted, so please ensure you have at least a back up copy oif your data.How do听I acknowledge 果冻影院 Genomics in my publication?
It is really important for the facility that our work is acknowledged in all publications.听For projects where we have worked with you on a collaborative basis (such as for custom analysis or use of in-house designed pathogen capture probes), we would expect to be co-authors on publications
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